Review



rabbit anti α4 gaba a r  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Alomone Labs rabbit anti α4 gaba a r
    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) <t>GABA</t> <t>A</t> <t>R</t> <t>α1-α4</t> subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Rabbit Anti α4 Gaba A R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+%CE%B14+gaba+a+r/Anti-GABA(A)+alpha4+Receptor+(extracellular)+Antibody/bio_rxiv__462457-177-34-32
    Average 92 stars, based on 7 article reviews
    rabbit anti α4 gaba a r - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors"

    Article Title: Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors

    Journal: bioRxiv

    doi: 10.1101/462457

    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Figure Legend Snippet: (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Techniques Used: Southern Blot, Mutagenesis, DNA Sequencing, Amplification, Sequencing, Expressing, Western Blot, Isolation, Quantitation Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors
    Article Snippet: The following horseradish-peroxidase-conjugated secondary antibodies (IgG(H&L)) were used: goat anti-rabbit (1:10000, Rockland Immunochemicals), goat anti-mouse (1:10000, Rockland Immunochemicals), donkey anti-guinea-pig (1:1000, Jackson Immunoresearch Laboratories). .. For immunohistochemistry, the following primary antibodies were used: rabbit anti-α1-GABA A R (1:20000, from Jean-Marc Fritschy, University of Zurich), guinea-pig anti-α2-GABA A R (1:1000, from Jean-Marc Fritschy), rabbit anti-α3-GABA A R (1:1000, Alomone Labs), rabbit anti-α4-GABA A R (1:500, Werner Sieghart), rabbit anti-α5-GABA A R (1:500, Werner Sieghart). .. Alexa Fluor 555-conjugated goat anti-rabbit and goat anti-guinea pig (IgG (H&L)) were purchased from Invitrogen Ltd and used at 1:2000.



    Similar Products

    rabbit anti α4 gaba a r  (Alomone Labs)
    92
    Alomone Labs rabbit anti α4 gaba a r
    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) <t>GABA</t> <t>A</t> <t>R</t> <t>α1-α4</t> subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Rabbit Anti α4 Gaba A R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+%CE%B14+gaba+a+r/Anti-GABA(A)+alpha4+Receptor+(extracellular)+Antibody/bio_rxiv__462457-177-34-32
    Average 92 stars, based on 1 article reviews
    rabbit anti α4 gaba a r - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Journal: bioRxiv

    Article Title: Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors

    doi: 10.1101/462457

    Figure Lengend Snippet: (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Article Snippet: For immunohistochemistry, the following primary antibodies were used: rabbit anti-α1-GABA A R (1:20000, from Jean-Marc Fritschy, University of Zurich), guinea-pig anti-α2-GABA A R (1:1000, from Jean-Marc Fritschy), rabbit anti-α3-GABA A R (1:1000, Alomone Labs), rabbit anti-α4-GABA A R (1:500, Werner Sieghart), rabbit anti-α5-GABA A R (1:500, Werner Sieghart).

    Techniques: Southern Blot, Mutagenesis, DNA Sequencing, Amplification, Sequencing, Expressing, Western Blot, Isolation, Quantitation Assay